The Khan Lab investigates mechanisms linking inflammation, autophagy, and carcinogenesis in patients with colorectal cancer, focusing on colitis and environmental exposures such as burn-pit toxins. Using murine models, organoids, and advanced molecular techniques, we aim to define how innate immune sensors like cGAS regulate epithelial and immune responses during inflammation-associated colorectal cancer development.

We are seeking a Laboratory Research Technician IV to join our translational research team. The ideal candidate will be experienced in molecular biology, histology, and cell-based assays, capable of managing complex workflows independently, and comfortable in both mouse and human tissue research settings.

Key Responsibilities

Perform and oversee molecular and cellular experiments, including DNA/RNA extraction, PCR, Western blotting, and ELISA

Conduct murine studies including genotyping, sample collection (colon, lymphoid organs), tissue processing, and histology preparation (FFPE, frozen)

Establish and maintain primary epithelial and immune cell cultures, including murine and human colonic organoids, and perform co-culture and drug treatment assays

Execute and assist with immunofluorescence and immunohistochemistry

Assist in flow cytometry and single-cell RNA sequencing sample preparation

Maintain detailed electronic lab notebooks and organize experimental data for analysis and publication

Support the PI and trainees in data analysis, figure preparation, and manuscript writing

Manage laboratory inventory, ordering, and compliance with safety protocols; serve as lab safety liaison as needed

Train and mentor junior staff, students, or fellows in lab techniques

Preferred Skills and Experience

Bachelor's or Master's degree in biological sciences with at least 3 years of laboratory experience, preferably in academic research

Proficiency in cell culture and organoid systems, including sterile technique, cryopreservation, and passaging

Experience with murine handling, tissue harvesting, sample preparation for histology and molecular assays

Hands-on experience with microscopy, immunofluorescence, and image analysis software (ImageJ, QuPath, or similar)

Experience with omics workflows (scRNA-seq, spatial transcriptomics, or related bioinformatics) is an advantage

Excellent organization, recordkeeping, and communication skills; ability to work both independently and collaboratively

The Khan Lab investigates mechanisms linking inflammation, autophagy, and carcinogenesis in patients with colorectal cancer, focusing on colitis and environmental exposures such as burn-pit toxins. Using murine models, organoids, and advanced molecular techniques, we aim to define how innate immune sensors like cGAS regulate epithelial and immune responses during inflammation-associated colorectal cancer development.

We are seeking a Laboratory Research Technician IV to join our translational research team. The ideal candidate will be experienced in molecular biology, histology, and cell-based assays, capable of managing complex workflows independently, and comfortable in both mouse and human tissue research settings.

Key Responsibilities

Perform and oversee molecular and cellular experiments, including DNA/RNA extraction, PCR, Western blotting, and ELISA.

Conduct murine studies including genotyping, sample collection (colon, lymphoid organs), tissue processing, and histology preparation (FFPE, frozen).

Establish and maintain primary epithelial and immune cell cultures, including murine and human colonic organoids, and perform co-culture and drug treatment assays.

Execute and assist with immunofluorescence and immunohistochemistry.

Assist in flow cytometry and single-cell RNA sequencing sample preparation.

Maintain detailed electronic lab notebooks and organize experimental data for analysis and publication.

Support the PI and trainees in data analysis, figure preparation, and manuscript writing.

Manage laboratory inventory, ordering, and compliance with safety protocols; serve as lab safety liaison as needed.

Train and mentor junior staff, students, or fellows in lab techniques.